The main focus of our group is in a development of the new experimental approaches and applications that will bring our original method of photofragmentation UV spectroscopy- highly resolution mass spectroscopy of cryogenically cooled ions to the needs of life science.
2D UV-MS photo fragmentation fingerprinting has demonstrates its high selectivity in identifications of a broad range of molecules. This invention allows qualitative and quantitative identification of isomers and conformers of aromatic peptides, drug molecules, saccharides, etc., using a library-based and a “blind” decomposition approaches. The foreseen applications of the method include, for instance, the doping and drug-related isomeric molecules, identification of point mutations in amyloid-forming and cancer proteins.
The second line of our research addresses a fundamental question of the relation between the gas-phase and the native (solution phase) structure of biomolecules. A bridge between the two phases is to study biomolecules solvated by a few solvent molecules (e.g., water) in the gas phase. We are developing an original method that may preserve the native structure of a molecule in such water clusters, but enables high resolution and selectivity of the gas-phase methods of spectroscopy and mass spectrometry to reveal this structure.
2. Ujma, J., Kopysov, V., Nagornova, N. S., Migas, L., Lizio, M. G., Blanch, E. W., MacPhee, C., Boyarkin, O. V. and Barran, P. E. Initial steps of amyloidogenic peptide assembly revealed by cold ion spectroscopy. Angew. Chem. Int. Ed. 2017, doi:10.1002/anie.201710188